褪黑素首批化学对照品的研制

王昆, 吴先富, 马玲云, 肖新月

中国药学杂志 ›› 2015, Vol. 50 ›› Issue (13) : 1142-1145.

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中国药学杂志 ›› 2015, Vol. 50 ›› Issue (13) : 1142-1145. DOI: 10.11669/cpj.2015.13.015
·论 著·

褪黑素首批化学对照品的研制

  • 王昆, 吴先富, 马玲云*, 肖新月
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Preparation and Standardization of Reference Standards of Melatonin

  • WANG Kun, WU Xian-fu , MA Ling-yun*, XIAO Xin-yue
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摘要

目的 建立供含量测定用褪黑素的首批化学对照品。方法 应用紫外光谱、红外光谱、核磁共振和质谱对褪黑素对照品进行结构鉴定,并采用HPLC进行纯度分析和含量测定。结果 HPLC测定本品含量为99.8%。结论 本实验中褪黑素对照品的建立为保健食品中添加褪黑素的检测和监督提供有力支持。

Abstract

OBJECTIVE To establish the reference standard of melatonin. METHODS The structure of the reference standard was identified by UV, IR, NMR, and ESI-MS spectrophotometry. The purity was calculated by normalization method and self-contrasted dilution method. Furthermore, loss on drying, residue on ignition, and hygroscopicity were tested. RESULTS The purity was 99.8% by normalization method and self-contrasted dilution method. COCLUSION The establishment of reference standards of melatonin can efficiently control the addtion of melatonin into functional foods.

关键词

褪黑素 / 对照品 / 结构鉴定 / 有关物质 / 含量测定

Key words

melatonin / reference standard / structure determination / related substance / content assay

引用本文

导出引用
王昆, 吴先富, 马玲云, 肖新月. 褪黑素首批化学对照品的研制[J]. 中国药学杂志, 2015, 50(13): 1142-1145 https://doi.org/10.11669/cpj.2015.13.015
WANG Kun, WU Xian-fu , MA Ling-yun, XIAO Xin-yue. Preparation and Standardization of Reference Standards of Melatonin[J]. Chinese Pharmaceutical Journal, 2015, 50(13): 1142-1145 https://doi.org/10.11669/cpj.2015.13.015
中图分类号: R917   

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基金

国家质量监督检验检疫总局课题资助项目(2012104008-1-12)
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